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Course profile

Laboratory Skills in Genetic Research (BIOL3380)

Study period
Sem 1 2025
Location
St Lucia
Attendance mode
In Person

Course overview

Study period
Semester 1, 2025 (24/02/2025 - 21/06/2025)
Study level
Undergraduate
Location
St Lucia
Attendance mode
In Person
Units
2
Administrative campus
St Lucia
Coordinating unit
The Environment School

In this intensive practical course, students will develop core genetic skills and will be given the opportunity to work independently within a supported scaffold. The course will be taught at a high level with students expected to take responsibility for all aspects of their practical work, much as would be required in a research or commercial lab. The core skills will be based around genetic research skills, with an additional emphasis on identifying and describing transferable skills relevant to future careers. Students will be required to make their stock solutions, calculate all dilutions, and conduct detailed trouble-shooting of results. Knowledge and experience gained at these core laboratory genetics skills will set students up well either for molecular laboratory-based employment or continuing study at honours level. Students will be mentored in the appropriate use of a laboratory notebook, as well as in lab safety and risk assessment. Students will be expected to present their results in descriptive, detailed and accurate reports. The advanced practical experiences will be designed around ongoing genetics research in the School of the Environment. Quota 48 (in total). Preference will given to students who have completed the pre-requisite course (BIOL2202/BIOL2902).

Course requirements

Assumed background

We assume that you have doneᅠsecond year genetics and will build on that knowledge.

Prerequisites

You'll need to complete the following courses before enrolling in this one:

BIOL2202 or BIOL2902

Recommended companion or co-requisite courses

We recommend completing the following courses at the same time:

BIOL3209, BIOL3004, BIOL3350

Restrictions

Maximum quota of 48

Course contact

Course staff

Lecturer

Tutor

Ms Angelique Asselin
Mr Brian Kim
Ms Leanne Sherrington
Mr Tyson Thomson

Timetable

The timetable for this course is available on the UQ Public Timetable.

Additional timetable information

Practicals run weekly every Tuesday starting in week 1 through to week 12. Attendance at all practicals is essential.

Aims and outcomes

In this intensive practical course, students will develop core genetic skills and will be given the opportunity to work independently within a supported scaffold. The course will be taught at a high level with students expected to take responsibility for all aspects of their practical work, much as would be required in a research or commercial lab. The core skills will be based around genetic research skills, with an additional emphasis on identifying and describing transferable skills relevant to future careers. Students will be required to make their stock solutions, calculate all dilutions, and conduct detailed trouble-shooting of results. Knowledge and experience gained at these core laboratory genetics skills will set students up well either for molecular laboratory-based employment or continuing study at honours level. Students will be mentored in the appropriate use of a laboratory notebook, as well as in lab safety and risk assessment. Students will be expected to present their results in descriptive, detailed and accurate reports. The advanced practical experiences will be designed around ongoing genetics research in the School of the Environment.

Learning outcomes

After successfully completing this course you should be able to:

LO1.

Understand practical aspects of a broad range of subfields within genetics

LO2.

Gain useful skills for the workplace

LO3.

Collect and interpret data and communicate the results

LO4.

Work collaboratively

LO5.

Work independently

LO6.

Troubleshoot and resolve unexpected problems

LO7.

define a problem and develop and implement solutions

LO8.

understand how to interpret and display data with integrity

Assessment

Assessment summary

Category Assessment task Weight Due date
Examination Experimental PCR exam
  • Identity Verified
  • In-person
25%

25/03/2025

Paper/ Report/ Annotation Technical research report 20%

1/05/2025 2:00 pm

Paper/ Report/ Annotation Formal research paper 20%

30/05/2025 2:00 pm

Examination Final exam
  • Hurdle
  • Identity Verified
  • In-person
35%

End of Semester Exam Period

7/06/2025 - 21/06/2025

A hurdle is an assessment requirement that must be satisfied in order to receive a specific grade for the course. Check the assessment details for more information about hurdle requirements.

Assessment details

Experimental PCR exam

  • Identity Verified
  • In-person
Mode
Written
Category
Examination
Weight
25%
Due date

25/03/2025

Other conditions
Time limited.

See the conditions definitions

Task description

This exam will be held in class. It will cover the first 4 weeks including calculating concentrations and dilutions, troubleshooting results from a PCR experiment, primer design, temperature cycling conditions.

This assessment task is to be completed in-person. The use of generative Artificial Intelligence (AI) or Machine Translation (MT) tools will not be permitted. Any attempted use of AI or MT may constitute student misconduct under the Student Code of Conduct.

Exam details

Planning time no planning time minutes
Duration 60 minutes
Calculator options

(In person) Casio FX82 series only or UQ approved and labelled calculator

Open/closed book Closed Book examination - no written materials permitted
Exam platform Paper based
Invigilation

Invigilated in person

Submission guidelines

Deferral or extension

You may be able to defer this exam.

See the Additional assessment information section further below for information relating to extension and deferral applications

Technical research report

Mode
Written
Category
Paper/ Report/ Annotation
Weight
20%
Due date

1/05/2025 2:00 pm

Learning outcomes
L01, L02, L03, L05, L06, L07, L08

Task description

Clarity of communication is essential! Having the ability to write in a simple and clear manner is a very valuable gift. In this task you will take data you have collected in class, analyse the outcomes and write a technical report.

This assessment task evaluates students' abilities, skills and knowledge without the aid of generative Artificial Intelligence (AI) or Machine Translation (MT). Students are advised that the use of AI or MT technologies to develop responses is strictly prohibited and may constitute student misconduct under the Student Code of Conduct.

Submission guidelines

Submit electronically through Turnitin. No hard copy or assignment coversheet required. Please keep your email receipt as proof of submission.

Deferral or extension

You may be able to apply for an extension.

The maximum extension allowed is 28 days. Extensions are given in multiples of 24 hours.

See the Additional assessment information section further below for information relating to extension and deferral applications

Late submission

A penalty of 10% of the maximum possible mark will be deducted per 24 hours from time submission is due for up to 7 days. After 7 days, you will receive a mark of 0.

You are required to submit assessable items on time. If you fail to meet the submission deadline for any assessment item, then 10% of the maximum possible mark for the assessment item (assessment ‘marked from’ value) will be deducted as a late penalty for every day (or part day) late after the due date. For example, if you submit your assignment 1 hour late, you will be penalised 10%; if your assignment is 24.5 hours late, you will be penalised 20% (because it is late by one 24-hour period plus part of another 24-hour period). 

Formal research paper

Mode
Written
Category
Paper/ Report/ Annotation
Weight
20%
Due date

30/05/2025 2:00 pm

Learning outcomes
L01, L02, L03, L04, L06, L07, L08

Task description

Published research papers are the primary medium though which scientific research information is exchanged. Typically, an important gap in knowledge is identified and investigated resulting in discoveries that lead to new understanding. Clarity of communication is essential! Having the ability to write in a simple and engaging manner is a very valuable gift. 

Scientific research papers usually adhere to a very defined structure. By following this structure, any reader of your manuscript automatically knows where to look to find specific information. The defined structure usually includes strict word or page limits. During the course of this module, we will progbress through the writing of a scientific manuscript in stages. At the end of the module, you will submit the completed, polished and perfected manuscript.

This assessment task evaluates students' abilities, skills and knowledge without the aid of generative Artificial Intelligence (AI) or Machine Translation (MT). Students are advised that the use of AI or MT technologies to develop responses is strictly prohibited and may constitute student misconduct under the Student Code of Conduct.

Submission guidelines

Submit electronically through Turnitin. No hard copy or assignment coversheet required. Please keep your email receipt as proof of submission.

Deferral or extension

You may be able to apply for an extension.

The maximum extension allowed is 28 days. Extensions are given in multiples of 24 hours.

See the Additional assessment information section further below for information relating to extension and deferral applications

Late submission

A penalty of 10% of the maximum possible mark will be deducted per 24 hours from time submission is due for up to 7 days. After 7 days, you will receive a mark of 0.

You are required to submit assessable items on time. If you fail to meet the submission deadline for any assessment item, then 10% of the maximum possible mark for the assessment item (assessment ‘marked from’ value) will be deducted as a late penalty for every day (or part day) late after the due date. For example, if you submit your assignment 1 hour late, you will be penalised 10%; if your assignment is 24.5 hours late, you will be penalised 20% (because it is late by one 24-hour period plus part of another 24-hour period). 

Final exam

  • Hurdle
  • Identity Verified
  • In-person
Mode
Written
Category
Examination
Weight
35%
Due date

End of Semester Exam Period

7/06/2025 - 21/06/2025

Learning outcomes
L01, L02, L06, L07, L08

Task description

This exam focusses on problem solving and utilisation of information and data rather than recall of facts. 

Hurdle requirements

You must obtain an overall mark of 45% or more in the end of semester exam. If you do not meet these requirements, the maximum grade you will receive will be a 3.

Exam details

Planning time 10 minutes
Duration 120 minutes
Calculator options

(In person) Casio FX82 series only or UQ approved and labelled calculator

Open/closed book Closed Book examination - no written materials permitted
Exam platform Paper based
Invigilation

Invigilated in person

Submission guidelines

Deferral or extension

You may be able to defer this exam.

See the Additional assessment information section further below for information relating to extension and deferral applications

Course grading

Full criteria for each grade is available in the Assessment Procedure.

Grade Description
1 (Low Fail)

Absence of evidence of achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 0%

2 (Fail)

Minimal evidence of achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 30%

3 (Marginal Fail)

Demonstrated evidence of developing achievement of course learning outcomes

Course grade description: The minimum percentage required for this grade is: 45%

4 (Pass)

Demonstrated evidence of functional achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 50%

5 (Credit)

Demonstrated evidence of proficient achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 65%

6 (Distinction)

Demonstrated evidence of advanced achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 75%

7 (High Distinction)

Demonstrated evidence of exceptional achievement of course learning outcomes.

Course grade description: The minimum percentage required for this grade is: 85%

Supplementary assessment

Supplementary assessment is available for this course.

Should you fail a course with a grade of 3, you may be eligible for supplementary assessment. Refer to my.UQ for information on supplementary assessment and how to apply.

Supplementary assessment provides an additional opportunity to demonstrate you have achieved all the required learning outcomes for a course.

If you apply and are granted supplementary assessment, the type of supplementary assessment set will consider which learning outcome(s) have not been met.

Supplementary assessment can take any form (such as a written report, oral presentation, examination or other appropriate assessment) and may test specific learning outcomes tailored to the individual student, or all learning outcomes.

To receive a passing grade of 3S4, you must obtain a mark of 50% or more on the supplementary assessment.

Additional assessment information

Submission of Assignments

Submission of assignments by email will NOT be accepted.

It is the responsibility of the student to ensure the on time, correct and complete submission of all assessment items.ᅠ

Please ensure you receive and save the submission confirmation for all submitted items, you may be asked to produce this as evidence of your submission.ᅠ

Turnitinᅠ

All written assessment must be submitted via the appropriate Turnitin submission portal, which can be found within the Blackboard site. You are responsible for ensuring that your submission is complete. It is wise to re-enter the Turnitin portal and confirm that your submission is there and that it has not been altered during the submission process.ᅠ

By submitting work through Turnitin you are deemed to have accepted the following declaration “I certify that this assignment is my own work and has not been submitted, either previously or concurrently, in whole or in part, to this University or any other educational institution, for marking or assessment”.ᅠ

In the case of a Blackboard outage, please contact the Course Coordinator as soon as possible to confirm the outage with ITS.ᅠ

Plagiarism

You should be aware that the University employs purpose built software to detect plagiarism. It is very important that you understand clearly the practical meaning of plagiarism.

DEFINITION OF PLAGIARISM: Plagiarism is the act of misrepresenting as one's own original work the ideas, interpretations, words or creative works of another. These include published and unpublished documents, designs, music, sounds, images, photographs, computer codes and ideas gained through working in a group. These ideas, interpretations, words or works may be found in print and/or electronic media.

EXAMPLES OF PLAGIARISM:

1. Direct copying of paragraphs, sentences, a single sentence or significant parts of a sentence;

2. Direct copying of paragraphs, sentences, a single sentence or significant parts of a sentence with an end reference but without quotation marks around the copied text;

3. Copying ideas, concepts, research results, computer codes, statistical tables, designs, images, sounds or text or any combination of these;

4. Paraphrasing, summarising or simply rearranging another person's words, ideas, etc without changing the basic structure and/or meaning of the text;

5. Offering an idea or interpretation that is not one's own without identifying whose idea or interpretation it is;

6. A 'cut and paste' of statements from multiple sources;

7. Presenting as independent, work done in collaboration with others;

8. Copying or adapting another student's original work into a submitted assessment item.

Artificial Intelligence use (AI)

The assessment tasks in this course evaluate students’ abilities, skills, and knowledge without the aid of Artificial Intelligence (AI).

Students are advised that the use of AI technologies to develop responses is strictly prohibited and may constitute misconduct under the Student Code of Conduct.

Applications for Extensions to Assessment Due Dates

Extension requests are submitted online via my.UQ – applying for an extension. Extension requests received in any other way will not be approved. Additional details associated with extension requests, including acceptable and unacceptable reasons, may be found at my.UQ.

Please note:

  • Requests for an extension to an assessment due date must be submitted through your my.UQ portal and you must provide documentation of your circumstances, as soon as it becomes evident that an extension is needed. Your application must be submitted on or before the assessment item's due date and time.
  • Applications for extension can take time to be processed so you should continue to work on your assessment item while awaiting a decision. We recommend that you submit any completed work by the due date, and this will be marked if your application is not approved. Should your application be approved, then you will be able to resubmit by the agreed revised due date.
  • If an extension is approved, you will be notified via your my.UQ portal and the new date and time for submission provided. It is important that you check the revised date as it may differ from the date that you requested.
  • If the basis of the application is a medical condition, applications should be accompanied by a medical certificate dated prior to the assignment due date. If you are unable to provide documentation to support your application by the due date and time you must still submit your application on time and attach a written statement (Word document) outlining why you cannot provide the documentation. You must then upload the documentation to the portal within 24 hours.
  • If an extension is being sought on the basis of exceptional circumstances, it must be accompanied by supporting documentation (eg. Statutory declaration).
  • For extensions based on a SAP you may be granted a maximum of 7 days (if no earlier maximum timeframe applies). See the Extension or Deferral availability section of each assessment for timeframes. Your SAP is all that is required as documentation to support your application. However, additional extension requests for the assessment item will require the submission of additional supporting documentation e.g., a medical certificate. All extension requests must be received by the assessment due date and time.
  • An extension for an assessment item due within the teaching period in which the course is offered, must not exceed four weeks in total. If you are incapacitated for a period exceeding four weeks of the teaching period, you are advised to apply for Removal of Course.
  • If you have been ill or unable to attend class for more than 4 weeks, you are advised to carefully consider whether you are capable of successfully completing your courses this semester. You might be eligible to withdraw without academic penalty - seek advice from the Faculty that administers your program.
  • Students may be asked to submit evidence of work completed to date. Lack of adequate progress on your assessment item may result in an extension being denied.
  • There are no provisions for exemption from an assessment item within UQ rules. If you are unable to submit an assessment piece then, under special circumstances, you may be granted an exemption but may be required to submit alternative assessment to ensure all learning outcomes are met.

Applications to defer an exam

In certain circumstances you can apply to take a deferred examination for in-semester and end-of-semester exams. You'll need to demonstrate through supporting documentation how unavoidable circumstances prevented you from sitting your exam. If you can’t, you can apply for a one-off discretionary deferred exam.

Deferred Exam requests are submitted online via mySi-net. Requests received in any other way will not be approved. Additional details associated with deferred examinations, including acceptable and unacceptable reasons may be found at my.UQ.

Please note:

  • Applications can be submitted no later than 5 calendar days after the date of the original exam.
  • There are no provisions to defer a deferred exam. You need to be available to sit your deferred examination.
  • Your deferred examination request(s) must have a status of "submitted" in mySI-net to be assessed.
  • All applications for deferred in-semester examinations are assessed by the relevant school. Applications for deferred end-of-semester examinations are assessed by the Academic Services Division.
  • You’ll receive an email to your student email account when the status of your application is updated.
  • If you have a medical condition, mental health condition or disability and require alternative arrangements for your deferred exam you’ll need to complete the online alternative exam arrangements through my.UQ. This is in addition to your deferred examinations request. You need to submit this request on the same day as your request for a deferred exam or supplementary assessment. Contact Student Services if you need assistance completing your alternative exam arrangements request.

Learning resources

You'll need the following resources to successfully complete the course. We've indicated below if you need a personal copy of the reading materials or your own item.

Library resources

Library resources are available on the UQ Library website.

Other course materials

If we've listed something under further requirement, you'll need to provide your own.

Required

Item Description Further Requirement
Lab coat own item needed
Safety glasses own item needed
Closed in shoes own item needed

Learning activities

The learning activities for this course are outlined below. Learn more about the learning outcomes that apply to this course.

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Learning period Activity type Topic
Multiple weeks
General contact hours

Mod 3 - Discovery Science

Define an hypothesis.
Design a mini research project to answer the question posed by your hypothesis in 2 different ways.
Statistical evaluation.
Analyse and display your results.
Interpret your results.
Write a mini scientific paper.
Research risk assessment.
Lecturer: Paul Ebert
Readings/Ref: Sci Writing1 (How to write a science report); Sci Writing2 (How to write a scientific abstract); Sci Writing 3 (How to present data); Mod 2 ; C. elegans 1 (How-to guide for C. elegans);

Learning outcomes: L02, L03, L04, L05, L06, L07, L08

Multiple weeks

From Week 1 To Week 5
(24 Feb - 30 Mar)

Practical

Mod 1-PCR immersion experience

PCR immersion experience and transferable skill development
In module 1 students will focus on development of skills critical to scientific analysis . The module will be centred around using the technique of PCR to identify and hone skills involved in the planning and implementation of an experiment and the analysis, trouble shooting and reporting of experimental results. During the module students will reflect on the experimental processes and explicitly identify the transferable skills that they are developing.
Lecturer: Karyn Johnson

Learning outcomes: L01, L02, L03, L05, L06, L07, L08

Week 1

(24 Feb - 02 Mar)

Practical

Week 1

Course overview (mini-lecture)

Careers in science and the importance of soft skills (Discussion)

PCR the process and experimental methods (Discussion)

Designing PCR primers (Practical)

Lab safety and induction

Learning outcomes: L01, L02, L05, L07

Week 2

(03 Mar - 09 Mar)

Practical

Week 2

  • Experimental design and planning (tutorial)
  • Science careers panel (discussion)
  • Preparation of buffers and reagents (tutorial and practical)
  • Individually perform DNA extraction and PCR with own primers (Practical)
  • Week 4

    (17 Mar - 23 Mar)

    Practical

    Week 3

  • Electrophoresis of PCR products (Practical)
  • Trouble shooting (mini-lecture)
  • Analysis of PCR results and trouble shooting; repeat PCR as necessary (practical)
  • Managing logistics (mini-lecture and discussion)
  • The importance of collaboration (discussion)
  • Learning outcomes: L01, L02, L03, L05, L06, L07, L08

    Week 5

    (24 Mar - 30 Mar)

    Practical

    Week 4

  • Complete PCR analysis
  • How to present scientific results in formal or semi-formal environments (discussion)
  • Discussion on outcomes of Module 1 (discussion)
  • Learning outcomes: L02, L03, L05, L06, L08

    Multiple weeks

    From Week 6 To Week 8
    (31 Mar - 20 Apr)

    Practical

    Mod 2 - Analysis and validation

    This module is primarily project based in which students will initially learn about various ways to determine differential gene expression when two or more treatments/conditions are compared. A powerful and commonly used technique is quantitative reverse transcription PCR (qRT-PCR). The theory, experimental design and application of the technique will be discussed, and to put it into practice, in this mini-project, you will be involved in the experimental design, gene selection, primer design, and trouble shootings in validating published deep sequencing data from blood-fed and non-blood fed mosquito transcriptomes centred around qRT-PCR.
    Building further on your acquired knowledge in the first module, you will develop skills in experimental planning and design and troubleshooting, extracting data from published sources, data analysis and interpretation, and finally communicating your experimental results in the form of a technical report.
    Lecturer: Sassan Asgari
    Readings/Ref: Scientific paper and notes provided throughout the module that will be uploaded on Bb.

    Learning outcomes: L01, L02, L03, L04, L05, L06, L07, L08

    Week 6

    (31 Mar - 06 Apr)

    Practical

    Week 6 - Starting the experiments

    Discussion on differential gene expression and approaches in measuring and validating differential gene expression (qRT-PCR)
    Whatメs involved in conducting your mini-project: designing experiment and primers, RNA extraction

    Learning outcomes: L01, L02, L03, L04, L05, L06, L07

    Week 7

    (07 Apr - 13 Apr)

    Practical

    Week 7

    Reverse transcription, validation of primers, trouble shooting, preparing qPCR plan

    Learning outcomes: L01, L02, L03, L04, L05, L06, L07, L08

    Week 8

    (14 Apr - 20 Apr)

    Practical

    Week 8

    qPCR, data analysis, trouble shooting, report writing

    Learning outcomes: L01, L02, L03, L04, L05, L06, L07, L08

    Week 9

    (28 Apr - 04 May)

    Practical

    Week 9a - Designing a discovery project


    How do you begin when you want to explore the unknown? (mini-lecture)
    Introduction to worm-handling protocols. (mini-lecture)
    Introduction to the experimental genetic "toolkit". (mini-lecture)
    Deciding on the hypothesis/research question. (discussion)
    HOMEWORK: Come to class next week with a research question that interests you and 2 approaches that you can use to address it. Also, read the Wormbook chapter "Maintenance of C. elegans". Write out the recipes for the medium you will need as well as the standard solutions. (You will be assessed on the medium and solutions homework next week, so get the assistance that you need this week.)

    Learning outcomes: L02, L07

    Practical

    Week 9b

    Preparing for the project & starting worm cultures
    Assessment: Check the experimental design homework. Check the medium and chemical solutions calculations. Are concentrations calculated properly. What is the solubility of the compounds that will be used?
    Proper use of a lab notebook. (mini-lecture)
    Fine tune your project with your tutor. (discussion)
    Make growth medium and solutions: autoclave or filter sterilise. (group by bench)
    Pour your growth medium plates. LABEL if supplemented with drugs or other chemicals. (individual)
    Seed your plates with bacteria. (individual)
    "Worm handling" (mini-lecture, Tim)
    Bleach the gravid hermaphrodites. (individual)
    Logistics (discussion)
    Plan your outside-of-class logistics with other members of your group.
    * Plate the L1 larvae
    HOMEWORK: None.

    Learning outcomes: L02, L04, L06

    Week 10

    (05 May - 11 May)

    Practical

    Week 10 - Starting the experiments


    Mfont color="green".Assessment: No homework due this week.
    Recap and feedback on lab work. (Paul & Tim)
    Plotting data using Excel & proper labelling of graphs. (mini-lecture)
    Pouring plates (individual)
    Seeding plates with bacteria (individual)
    bleach gravid hermaphrodites (individual)
    make medium for next week (can work in groups)
    Writing the introduction section of your paper (mini-lecture)
    Structure and content of the Materials & Methods section of a scientific paper (mini-lecture)
    Plan your outside-of-class logistics with other members of your group.
    * Plate the L1 larvae
    HOMEWORK: Write an introduction to your research paper that includes enough background information to provide the context for your research. Include references. Also, write the materials and methods section of your paper.
    Plot your initial data with Excel. Label the plot in a professional manner. Write a clear and descriptive figure legend.
    Readings/Ref: Sci Writing 3 (tables and figures); Sci Writing1 (focus results section);

    Learning outcomes: L02, L03, L04, L05, L06, L08

    Week 11

    (12 May - 18 May)

    Practical

    Week 11-Data collection,writing results,discussion

    Learning outcomes: L02, L03, L04, L05, L06, L08

    Week 12

    (19 May - 25 May)

    Practical

    Week 12 -Final C. elegans experimental week

    Learning outcomes: L02, L03, L04, L05, L06, L08

    Policies and procedures

    University policies and procedures apply to all aspects of student life. As a UQ student, you must comply with University-wide and program-specific requirements, including the:

    Learn more about UQ policies on my.UQ and the Policy and Procedure Library.